[annotation] Question about Isoform Annotation

[Kimberly Van Auken]

Hi--

I'm trying to decide the best way to annotate information about the C. 
elegans GABA receptor.

In C. elegans, functional GABA receptors are formed from one or more 
protein subunits encoded
by the same genomic locus, unc-49.

To test functionality, the authors of the paper expressed subunits 
separately and then together
and measured the dose-response to, and response to continuous 
application of, GABA.   One subunit
has receptor activity on its own, one does not, and expression of both 
results in a response distinct
from that of the first on its own.  To summarize:

    Subunit B:  responds

    Subunit C: doesn't respond

    Subunits B and C: respond with distinct profile


If this was just one isoform, I would annotate the protein to the 
appropriate receptor activity terms
using the IDA evidence code.  But is there any way to annotate this 
complexity using IDA?

I can certainly annotate to the B isoform, but I don't want to make the 
same annotation to the C isoform.

One way I can think to capture the complexity would be to annotate to 
both B and C, but include the
other isoform in the WITH column for one B annotation and the only C 
annotation.

Is the WITH column completely off-limits for IDA annotations?

Any thoughts on this would be much appreciated!

Thanks,
Kimberly