[annotation] annotation of ion channel subunits

[David Hill]

Hi Doug,

I have not been annotating in this way. I only annotate for the data 
presented in the paper. If we can't tell it has the contributes_to tag 
from the experiment, we don't use the tag. This is particularly common 
for mutants where a function is eliminated when you knock out any of the 
key subunits.

David


Doug howe wrote:
> For the Ref. Genomes we are annotating the gene p2rx3, a subunit of an 
> ATP activated cation channel.
> A paper I have shows that adding this gene to hek293 cells results in 
> the generation of an inward current in the presence of ATP.
>
> We know (not from this paper) that p2x receptors are a complex of 
> subunits.  Should this be annotated as 'contributes_to' ATP-gated 
> cation channel activity by IDA because it is thought to be part of an 
> ion channel complex, or is it not 'contributes_to' because you get the 
> current by introduction of just the single gene product (even if it is 
> forming a homomeric channel complex)??
>
> Introduction of both zebrafish p2rx3 and rat p2rx5 produces a channel 
> with novel properties....what can be done with that? 'protein 
> heterooligomerization' with the rat p2rx5 by IGI?
>
>
> -Doug