[annotation] annotation of ion channel subunits

[Doug howe]

So in one paper if one experiment was done with a single ion channel 
subunit and another was done with two ion channel subunits, producing 
different types of currents, would you have one contributes_to and one 
without contributes_to?
-Doug

David Hill wrote:
> Hi Doug,
>
> I have not been annotating in this way. I only annotate for the data 
> presented in the paper. If we can't tell it has the contributes_to tag 
> from the experiment, we don't use the tag. This is particularly common 
> for mutants where a function is eliminated when you knock out any of 
> the key subunits.
>
> David
>
>
> Doug howe wrote:
>> For the Ref. Genomes we are annotating the gene p2rx3, a subunit of 
>> an ATP activated cation channel.
>> A paper I have shows that adding this gene to hek293 cells results in 
>> the generation of an inward current in the presence of ATP.
>>
>> We know (not from this paper) that p2x receptors are a complex of 
>> subunits.  Should this be annotated as 'contributes_to' ATP-gated 
>> cation channel activity by IDA because it is thought to be part of an 
>> ion channel complex, or is it not 'contributes_to' because you get 
>> the current by introduction of just the single gene product (even if 
>> it is forming a homomeric channel complex)??
>>
>> Introduction of both zebrafish p2rx3 and rat p2rx5 produces a channel 
>> with novel properties....what can be done with that? 'protein 
>> heterooligomerization' with the rat p2rx5 by IGI?
>>
>>
>> -Doug
>
>