[Annotation] Fwd: [Evidence] ISS/ISA from Southerns?

[Rama Balakrishnan]

I haven't heard from anybody on this so far. Is it okay if we add  
'southern analysis' as an acceptable evidence to the ISS documentation?

Thanks,

Rama


Begin forwarded message:

> From: Rama Balakrishnan <rama at genome.stanford.edu>
> Date: May 9, 2008 5:18:06 PM PDT
> To: Evidence Code Group <evidence at genome.stanford.edu>
> Cc: GO Annotation list <annotation at genome.stanford.edu>
> Subject: [Evidence] ISS/ISA from Southerns?
>
> Reposting...
> I am dealing with a similar situation like the one Julie has  
> mentioned below.
>
> Thanks,
>
> Rama
>
>
> Begin forwarded message:
>> From: Julie Park <juliep at genome.stanford.edu>
>> Date: April 23, 2008 3:57:22 PM PDT
>> To: evidence at genome.stanford.edu
>> Subject: [Evidence] ISS/ISA from Southerns?
>>
>> Dear evidence code group,
>>
>> I had a question about whether Southern analysis alone can be used as
>> evidence for an annotation.
>>
>> In S. cerevisiae, MEL2-10 are annotated by ISS to alpha-galactosidase
>> activity.  There is no sequence alignment or information (older
>> literature, pre-genome sequencing), instead the authors infer  
>> homology/
>> sequence similarity from a Southern hybridization using a MEL1 probe.
>> Southern analysis was listed in the old ISS documentation but the  
>> only
>> mention of Southerns in the new documentation is under ISA in
>> conjunction with a sequence alignment.
>>
>> Is ISS by Southern only no longer allowed or did it just get left out
>> of the current documentation?
>> If allowed, would I leave these annotations as ISS or would it be  
>> okay
>> to ISA w/ MEL1?
>>
>> thanks,
>> Julie
>>
>> ****************************************************************
>> Julie Park, Ph.D., Scientific Curator
>>  Saccharomyces Genome Database
>>  Department of Genetics
>>  Stanford University School of Medicine
>>  Stanford, CA 94305-5120 USA
>>  phone: (650)724-9959  fax: (650)725-1534
>>  http://www.yeastgenome.org
>> *****************************************************************
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>
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