[Annotation] [Go] Evidence code for gfp and phoA fusions?

[David Hill]

Hi Jim,

I would use IDA. As long as the purpose of the experiment is to show the 
normal localization of the protein and the authors show no reason to 
believe that the tags mislocalize the protein, then the authors are 
looking at the normal distribution. Although these experiments are 
subject to mislocalization due to the tags, like all experiments are 
subject to artifact, I think we need to go with the authors conclusions.
I have annotated GFP fusions in mammalian cells using IDA.

David

Jim Hu wrote:
> We have a lot of annotations that would be made to intrinsic to 
> membrane based on expression of GFP and PhoA fusion proteins.  What 
> evidence code would people use?
>
> Jim
> =====================================
> Jim Hu
> Associate Professor
> Dept. of Biochemistry and Biophysics
> 2128 TAMU
> Texas A&M Univ.
> College Station, TX 77843-2128
> 979-862-4054
>
>
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