[Annotation] [Go] Evidence code for gfp and phoA fusions?

[David Hill]

It depends on the experiment. I didn't read the paper, but I assumed 
they showed the fusion protein was intrinsic to the membrane. If they 
didn't, then you are correct.

David

Pascale Gaudet wrote:
> But, can you really see 'intrinsic to the membrane' by GFP?
> I think you can only see that it's membrane, and you know it's intrinsic because 
> there are TM domains (which would be ISM).
> It's again one of those cases where the curator combines two different pieces of 
> information to make an annotation. We should think of better ways to deal with 
> this.
>
> Pascale
>
> David Hill wrote:
> > Hi Jim,
> >
> > I would use IDA. As long as the purpose of the experiment is to show the 
> > normal localization of the protein and the authors show no reason to believe 
> > that the tags mislocalize the protein, then the authors are looking at the 
> > normal distribution. Although these experiments are subject to mislocalization 
> > due to the tags, like all experiments are subject to artifact, I think we need 
> > to go with the authors conclusions.
> > I have annotated GFP fusions in mammalian cells using IDA.
> >
> > David
> >
> > Jim Hu wrote:
> >> We have a lot of annotations that would be made to intrinsic to membrane 
> >> based on expression of GFP and PhoA fusion proteins.  What evidence code 
> >> would people use?
> >>
> >> Jim
> >> =====================================
> >> Jim Hu
> >> Associate Professor
> >> Dept. of Biochemistry and Biophysics
> >> 2128 TAMU
> >> Texas A&M Univ.
> >> College Station, TX 77843-2128
> >> 979-862-4054
> >>
> >>
> >>
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