[Annotation] compartment annotation of a protein complex

[Ingrid Keseler]

Hi,

I have a question regarding how to annotate the localization of the 
components of a protein complex.  PMID 19368556 describes the YagTSR 
complex of E. coli.  Looks like they did size exclusion chromatography 
to show that it is a heterotrimer.  It also turns out that one of the 
components, YagT, normally contains a Tat signal sequence and has been 
experimentally shown to be transported to the periplasm.  So the 
question is, how would you annotate the presumably (and stated by the 
authors) periplasmic localization of the other two components of the 
complex?  Using NAS?  Using IC?  But "with" what?  Or IPI?  Something 
I'd be tempted to do, but pairwise interactions between YagS/R and YagT 
weren't shown.

I'm hoping that the answer is obvious to somebody... :-)

Thanks,
Ingrid