[Annotation] compartment annotation of a protein complex

[Harold Drabkin]

Ingrid Keseler wrote:
> Hi,
>
> I have a question regarding how to annotate the localization of the 
> components of a protein complex.  PMID 19368556 describes the YagTSR 
> complex of E. coli.  Looks like they did size exclusion chromatography 
> to show that it is a heterotrimer.  It also turns out that one of the 
> components, YagT, normally contains a Tat signal sequence and has been 
> experimentally shown to be transported to the periplasm.  So the 
> question is, how would you annotate the presumably (and stated by the 
> authors) periplasmic localization of the other two components of the 
> complex?  Using NAS?  Using IC?  But "with" what?  Or IPI?  Something 
> I'd be tempted to do, but pairwise interactions between YagS/R and 
> YagT weren't shown.
>
> I'm hoping that the answer is obvious to somebody... :-)
>
> Thanks,
> Ingrid
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always with the experiment; the other two are hearsay for this paper; If 
they don't give a reference to look at, then how do they know?