[Annotation] compartment annotation of a protein complex

[Rama Balakrishnan]

YagT could be targetted to periplasm by itself. There is no evidence  
that it goes there as a complex with the other two proteins. I  
wouldn't annotate the other two proteins to periplasm with any  
experimental ev-Code. NAS?


Rama

On Jun 24, 2009, at 12:52 PM, Ingrid Keseler wrote:

> Hi,
>
> I have a question regarding how to annotate the localization of the  
> components of a protein complex.  PMID 19368556 describes the YagTSR  
> complex of E. coli.  Looks like they did size exclusion  
> chromatography to show that it is a heterotrimer.  It also turns out  
> that one of the components, YagT, normally contains a Tat signal  
> sequence and has been experimentally shown to be transported to the  
> periplasm.  So the question is, how would you annotate the  
> presumably (and stated by the authors) periplasmic localization of  
> the other two components of the complex?  Using NAS?  Using IC?  But  
> "with" what?  Or IPI?  Something I'd be tempted to do, but pairwise  
> interactions between YagS/R and YagT weren't shown.
>
> I'm hoping that the answer is obvious to somebody... :-)
>
> Thanks,
> Ingrid
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