[Go] Advice on adding an enzyme function

[Alexander Diehl]

Jane,

I would vote no, if the following conditions are true:

1)  When presented with any of the eukaryotic substrates, the 
prokaryotic enzyme catalyzes the same reaction as the eukaryotic one.

2)  When presented with any of the prokaryotic substrates, the 
eukaryotic enzyme catalyzes the same reaction as the prokaryotic one.

When you say that the prokaryotic enzyme acts on a different range of 
alkane-alpha,omega-diamine substrates, do you mean that it has a 
different range of substrates available in the organisms where it is 
found, or that it cannot act upon the substances found in eukaryotic 
organisms.?

If the latter is true, I would vote yes, and create substrate specific 
terms for GO:0004145.

-- Alex


Jane Lomax wrote:
> Hi - do we have any guidelines anywhere for when to add an enzyme 
> function term or not? I had a look on the wiki and in the 
> documentation and couldn't see anything. If not, perhaps we should add 
> something - it seems that there have been quite a few discussions in 
> this area and might be good to capture it all somewhere.
>
> Anyhow, my dilemma is this - we have a term 'diamine 
> N-acetyltransferase activity ;  GO:0004145' which has EC 2.3.1.57. The 
> EC is based on the eukaryotic enzyme, and the reaction catalysed is: 
> acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine. 
> Several different substrates (i.e. alkane-alpha,omega-diamines) are 
> acted upon.
>
> The equivalent prokaryotic enzyme has essentially the same reaction, 
> *but* acts on a different range of alkane-alpha,omega-diamine substrates.
>
> So should I add a new term for the prokaryotic function or not?
>
> thanks,
>
> Jane
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-- 
Alexander Diehl, Ph.D.
Senior Scientific Curator
Mouse Genome Informatics
The Jackson Laboratory
600 Main Street
Bar Harbor, ME  04609

email:  adiehl at informatics.jax.org
work:  +1 (207) 288-6427
fax:  +1 (207) 288-6131