[Go] Advice on adding an enzyme function

[Jane Lomax]

Hi Alex - both the euk and prok enzymes act on spermine and spermidine, 
but the euk enzyme also acts on a range of other 
alkane-alpha,omega-diamine substrates including putrescine and 
1,3-diaminopropane. In an assay, the prok enzyme does not act on 
putrescine and 1,3-diaminopropane.

So I think you're suggesting I add child terms to GO:0004145, such as 
'spermine N-acetyltransferase activity' etc?

Jane


Alexander Diehl wrote:
> Jane,
>
> I would vote no, if the following conditions are true:
>
> 1)  When presented with any of the eukaryotic substrates, the 
> prokaryotic enzyme catalyzes the same reaction as the eukaryotic one.
>
> 2)  When presented with any of the prokaryotic substrates, the 
> eukaryotic enzyme catalyzes the same reaction as the prokaryotic one.
>
> When you say that the prokaryotic enzyme acts on a different range of 
> alkane-alpha,omega-diamine substrates, do you mean that it has a 
> different range of substrates available in the organisms where it is 
> found, or that it cannot act upon the substances found in eukaryotic 
> organisms.?
>
> If the latter is true, I would vote yes, and create substrate specific 
> terms for GO:0004145.
>
> -- Alex
>
>
> Jane Lomax wrote:
>> Hi - do we have any guidelines anywhere for when to add an enzyme 
>> function term or not? I had a look on the wiki and in the 
>> documentation and couldn't see anything. If not, perhaps we should 
>> add something - it seems that there have been quite a few discussions 
>> in this area and might be good to capture it all somewhere.
>>
>> Anyhow, my dilemma is this - we have a term 'diamine 
>> N-acetyltransferase activity ;  GO:0004145' which has EC 2.3.1.57. 
>> The EC is based on the eukaryotic enzyme, and the reaction catalysed 
>> is: acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an 
>> N-acetyldiamine. Several different substrates (i.e. 
>> alkane-alpha,omega-diamines) are acted upon.
>>
>> The equivalent prokaryotic enzyme has essentially the same reaction, 
>> *but* acts on a different range of alkane-alpha,omega-diamine 
>> substrates.
>>
>> So should I add a new term for the prokaryotic function or not?
>>
>> thanks,
>>
>> Jane
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>
>